Identification of Two Homologous Genes, chlAI and chlAII, That Are Differentially Involved in Isocyclic Ring Formation of Chlorophyll a in the Cyanobacterium Synechocystis sp. PCC 6803*

@article{Minamizaki2008IdentificationOT,
  title={Identification of Two Homologous Genes, chlAI and chlAII, That Are Differentially Involved in Isocyclic Ring Formation of Chlorophyll a in the Cyanobacterium Synechocystis sp. PCC 6803*},
  author={Kei Minamizaki and T. Mizoguchi and Takeaki Goto and H. Tamiaki and Y. Fujita},
  journal={Journal of Biological Chemistry},
  year={2008},
  volume={283},
  pages={2684 - 2692}
}
The isocyclic ring (E-ring) is a common structural feature of chlorophylls. The E-ring is formed by two structurally unrelated Mg-protoporphyrin IX monomethylester (MPE) cyclase systems, oxygen-dependent (AcsF), and oxygen-independent (BchE) systems, which involve incorporation of an oxygen atom from molecular oxygen and water into the C-131 position of MPE, respectively. Which system operates in cyanobacteria that can thrive in a variety of anaerobic environments remains an open question. The… Expand
Functional differentiation of two analogous coproporphyrinogen III oxidases for heme and chlorophyll biosynthesis pathways in the cyanobacterium Synechocystis sp. PCC 6803.
TLDR
Results suggested that sll1185 encodes HemF as the sole CPO under aerobic conditions and sll1876 encode HemN operating under micro-oxic conditions, together with HemF, which would ensure the stable supply of tetrapyrrole pigments under environments where oxygen levels fluctuate greatly. Expand
Synthesis of Chlorophyll-Binding Proteins in a Fully Segregated Δycf54 Strain of the Cyanobacterium Synechocystis PCC 6803
TLDR
The complete deletion of the ycf54 gene in the cyanobacterium Synechocystis 6803 is reported, and the greatly reduced formation of protochlorophyllide in the Δycf54 strain provided an opportunity to examine the synthesis of all known Chl-binding protein complexes under drastically restricted de novo Chl biosynthesis. Expand
Conserved residues in Ycf54 are required for protochlorophyllide formation in Synechocystis sp. PCC 6803
TLDR
It is concluded that the Ycf54 protein must form a stable interaction with CycI to promote optimal Pchlide biosynthesis. Expand
ChlR Protein of Synechococcus sp. PCC 7002 Is a Transcription Activator That Uses an Oxygen-sensitive [4Fe-4S] Cluster to Control Genes involved in Pigment Biosynthesis*
TLDR
It is concluded that ChlR is a transcriptional activator that uses a [4Fe-4S] cluster to sense O2 levels and thereby control the expression of the acsFII-ho2-hemN-desF operon. Expand
The role of psbA1 in Synechocystis sp. PCC 6803 under low-oxygen conditions
The transcriptional response of the model cyanobacterium Synechocystis sp. PCC 6803 to low-oxygen conditions includes the up-regulation of several clusters of genes related to photosynthesis andExpand
Identification of the chlE gene encoding oxygen-independent Mg-protoporphyrin IX monomethyl ester cyclase in cyanobacteria.
TLDR
It is reported that two bchE candidate genes from Cyanothece strains PCC 7425 and PCC 7822 restore the photosynthetic growth and bacteriochlorophyll production in a b chE-lacking mutant of Rhodobacter capsulatus. Expand
MarR-type Transcriptional Regulator ChlR Activates Expression of Tetrapyrrole Biosynthesis Genes in Response to Low-oxygen Conditions in Cyanobacteria*
TLDR
It is demonstrated that Sll1512 is an activator in response to low-oxygen environments and that the D35H variant becomes a constitutive activator. Expand
Conserved Chloroplast Open-reading Frame ycf54 Is Required for Activity of the Magnesium Protoporphyrin Monomethylester Oxidative Cyclase in Synechocystis PCC 6803
TLDR
Ycf54 is essential for the activity and stability of the oxidative cyclase, and levels of the cyclase component Sll1214 and the Chl biosynthesis enzymes Mg-protoporphyrin IX methyltransferase and protochlorophyllide reductase are significantly impaired in the ycf54 mutant. Expand
Gene expression under low-oxygen conditions in the cyanobacterium Synechocystis sp. PCC 6803 demonstrates Hik31-dependent and -independent responses.
TLDR
It is suggested that Hik31 helps to regulate growth and overall cellular homeostasis and regulates an important series of genes that relate to energy production and growth and that help to determine how Synechocystis responds to changes in O2 conditions. Expand
Differential requirement of two homologous proteins encoded by sll1214 and sll1874 for the reaction of Mg protoporphyrin monomethylester oxidative cyclase under aerobic and micro-oxic growth conditions.
TLDR
The findings indicate the essential contribution of CycI to the cyclase reaction at ambient and low oxygen conditions, whileLow oxygen conditions additionally require CycII for the cycl enzyme activity. Expand
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It is demonstrated that the B-ring of chlorin is reduced by a second nitrogenase-like enzyme and that the sequential actions of two nitrogen enzyme-like enzymes, DPOR and COR, convert porphyrin to bacteriochlorin. Expand
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A respiring culture of Rhodobacter sphaeroides, grown in the dark under defined aerobic conditions, produced cells capable of immediately commencing adaptation to photosynthetic growth on exposure toExpand
Differential Operation of Dual Protochlorophyllide Reductases for Chlorophyll Biosynthesis in Response to Environmental Oxygen Levels in the Cyanobacterium Leptolyngbya boryana1
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It is reported that anaerobic conditions are required for DPOR to compensate for the loss of LPOR in cyanobacterial cells, providing important concepts for understanding how dual Pchlide reductases operate differentially in oxygenic photosynthetic cells grown under natural environments where oxygen levels undergo dynamic changes. Expand
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TLDR
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TLDR
It is found that vitamin B(12)-requiring mutants of the bluE and bluB genes of Rhodobacter capsulatus, grown without B( 12), accumulated Mg-porphyrins and laser desorption/ionization time-of-flight MS and NMR spectroscopy identified them as MPE and its 3-vinyl-8-ethyl (mvMPE) derivative. Expand
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