Identification of Tumoricidal TCRs from Tumor-Infiltrating Lymphocytes by Single-Cell Analysis.

@article{Shitaoka2018IdentificationOT,
  title={Identification of Tumoricidal TCRs from Tumor-Infiltrating Lymphocytes by Single-Cell Analysis.},
  author={Kiyomi Shitaoka and Hiroshi Hamana and Hiroyuki Kishi and Yoshihiro Hayakawa and Eiji Kobayashi and Kenta Sukegawa and Xiuhong Piao and Fulian Lyu and Takuya Nagata and Daisuke Sugiyama and Hiroyoshi Nishikawa and Atsushi Tanemura and Ichiro Katayama and Mutsunori Murahashi and Yasushi Yokohama-shi Takamatsu and Kenzaburo Tani and Tatsuhiko Ozawa and Atsushi Muraguchi},
  journal={Cancer immunology research},
  year={2018},
  volume={6 4},
  pages={
          378-388
        }
}
T-cell receptor (TCR) gene therapy is a promising next-generation antitumor treatment. We previously developed a single-T-cell analysis protocol that allows the rapid capture of paired TCRα and β cDNAs. Here, we applied the protocol to analyze the TCR repertoire of tumor-infiltrating lymphocytes (TIL) of various cancer patients. We found clonally expanded populations of T cells that expressed the same clonotypic TCR in 50% to 70% of CD137+CD8+ TILs, indicating that they responded to certain… CONTINUE READING
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