Identification of Trichoderma spp. by DNA Barcode and Screening for Cellulolytic Activity

  title={Identification of Trichoderma spp. by DNA Barcode and Screening for Cellulolytic Activity},
  author={A. I. Fahmi and R. A. Eissa and Khalil A El-Halfawi and H. Hamza and M. Helwa},
  journal={Journal of Microbial \& Biochemical Technology},
Species identification of isolates of Trichoderma from different locations of Nile delta of Egypt was performed and their cellulolytic activities were analyzed. On the basis of morphological characteristics, 75% of isolates were identified to species level and they were divided into four aggregate groups. Morphological characterization alone was insufficient to precisely identify Trichoderma species because they have relatively few morphological characters and limited variation that cause… Expand

Figures and Tables from this paper

Genetic Characterization of Trichoderma spp. Isolated from Different Locations of Menoufia, Egypt and Assessment of their AntagonisticAbility
All 25 Trichoderma strains and isolates exhibited inhibition to the mycelial growth of four pathogenens and no correlation was found between total chitinolytic activities and total protein contents. Expand
The diversity of Trichoderma (Hypocreales, Ascomycota) species from Egypt and Saudi Arabia was achieved, and DNA barcoding of 21 strains based on the internal transcribed spacers 1 and 4 (ITS1 and 4)Expand
Comparative molecular genetic diversity between Trichoderma spp. from Egypt and Saudi Arabia
The results of the present study indicated that the Saudi Arabian isolates showed greater nucleotide diversity compared to Egyptian isolates, which will assist in future studies while assessing Trichoderma genetic diversity. Expand
Rapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence-characterized amplified region markers
To develop species-specific markers for detecting Trichoderma koningiopsis and T. longibrachiatum, the sequence-characterized amplified region technique, using 20 inter-simple sequence repeat-polymerase chain reaction primers, was performed. Expand
Influence of Protoplast Fusion in Trichoderma Spp. on Controlling SomeSoil Borne Diseases
The results in this study suggested that the protoplast fusion technique is useful for developing the superior hybrid strains and enhance antagonistic activity of Trichoderma spp. Expand
Internal transcribed spacers (ITS) based identification of Trichoderma isolates and biocontrol activity against Macrophomina phaseolina, Aspergillus niger and Meloidogyne incognita
Treatment with Trichoderma isolates improved tomato growth parameters (root length, plant height, roots and shoots fresh weight and shoots dry weight) and antagonism against Macrophomina phaseolina, Aspergillus niger, Meloidogyne incognita. Expand
Pathology, Ecology and Infection of E.coli
E. coli is a facultative, pole formed microbes that can be ordinarily found in creature dung, lower digestion systems of well evolved creatures, and even on the edge of hot springs, and can bring about a few intestinal and additional intestinal diseases. Expand


Molecular Characterization and Identification of Biocontrol Isolates of Trichoderma spp
Correlation between different genotypes and potential biocontrol activity was studied under dual culturing of 17 BCAs in the presence of the phytopathogenic fungi Phoma betae, Rosellinia necatrix, Botrytis cinerea, andFusarium oxysporum f. Expand
An oligonucleotide barcode for species identification in Trichoderma and Hypocrea.
It is concluded that oligonucleotide barcode is a powerful tool for the routine identification of Hypocrea/Trichoderma species and should be useful as a complement to traditional methods. Expand
PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes.
This is the first exhaustive study of fungal diversity at species level in vineyard by using restriction digestion analysis of the ITS products to identify isolates of filamentous fungi on grapes. Expand
Genetic relatedness of Trichoderma sect. Pachybasium species based on molecular approaches.
Molecular approaches including internal transcribed spacer (ITS) sequences of ribosomal DNA, universal primer polymerase chain reaction (UP-PCR) fingerprinting, and DNA-DNA hybridization were used toExpand
Phylogenetic diversity analysis of Trichoderma species based on internal transcribed spacer (ITS) marker
An integrated approach of morphological and molecular markers can be employed to identify a superior strain of Trichoderma for its commercial exploitation. Expand
Isolation and identification of Trichoderma species from different habitats and their use for bioconversion of solid waste
In testing for an efficient bioconversion agent, T. harzianum (IMI-392432) was found to be the most effective in kitchen waste decomposition and provided the highest volume and weight losses in waste treated with spore suspension. Expand
Species diversity of Trichoderma in Poland
The present study reinvestigate the diversity of Trichoderma in Poland utilizing a combination of morphological and molecular/phylogenetic methods and isolates belonging to the Viride clade are identified. Expand
Characterization of field isolates of Trichoderma antagonistic against Rhizoctonia solani.
This is the first report of an efficient antagonistic strain of T. gamsii being able to reduce the disease in different conditions and the ability to produce water-soluble inhibitors or coil around the hyphae of the pathogen in vitro was not related to the disease reduction in vivo. Expand
Molecular Characterization of Trichoderma viride and Trichoderma harzianum Isolated from Soils of North Bengal Based on rDNA Markers and Analysis of Their PCR-RAPD Profiles
ITS-PCR of rDNA region with ITS1 and ITS4 primers produced 600bp products in all isolates indicated the identification patterns of Trichoderma isolates. Expand
Phylogeny and evolution of the genus Trichoderma: a multigene approach
The results confirm the recent concept for sects Longibrachiatum and Trichoderma, indicate that the sects Hypocreanum and Pachybasium cannot be distinguished phylogenetically, and provide a first phylogenetic basis for dissection of the latter two sections. Expand