Identification of Three Genetic Risk Factors for Venous Thrombosis Using a Multiplex Allele-Specific PCR Assay: Comparison of Conventional and New Alternative Methods for the Preparation of DNA from Clinical Samples

@article{Angelini2003IdentificationOT,
  title={Identification of Three Genetic Risk Factors for Venous Thrombosis Using a Multiplex Allele-Specific PCR Assay: Comparison of Conventional and New Alternative Methods for the Preparation of DNA from Clinical Samples},
  author={Aantonio Angelini and Concetta Di Febbo and Giovanna Baccante and Marcello Di Nisio and Carmine Di Ilio and Franco Cuccurullo and Ettore Porreca},
  journal={Journal of Thrombosis and Thrombolysis},
  year={2003},
  volume={16},
  pages={189-193}
}
The demand for thrombophilia testing at the molecular level is increasing and with it the need for a simple and rapid and cost-saving procedure for the preparation of genomic DNA from whole blood samples. The aim of this paper is to compare the efficiency of two conventional commercial procedures (Genomic, Eurobio-Labtek, and Nucleospin, Macherey-Nagel) and two our alternative approaches (microwave irradiation and resin-binding method) for extraction DNA and their suitability and convenience… CONTINUE READING

References

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New method for the extraction of DNA from white blood cells for the detection of common genetic variants associated with thrombophilia

  • A Angelini, C Di Febbo, A Rullo, C Di Ilio, F Cuccurullo, E. Porreca
  • Pathophysiol Haemost Thromb 2002;32:180–183
  • 2002
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