Identification of Three Cationic Amino Acid Transporters in Placental Trophoblast: Cloning, Expression, and Characterization of hCAT-1

@article{Kamath1999IdentificationOT,
  title={Identification of Three Cationic Amino Acid Transporters in Placental Trophoblast: Cloning, Expression, and Characterization of hCAT-1},
  author={S. G. Kamath and T. Furesz and B. A. Way and C. Smith},
  journal={The Journal of Membrane Biology},
  year={1999},
  volume={171},
  pages={55-62}
}
Abstract. The concentrative transfer of amino acids from maternal to fetal blood is essential to fetal growth and metabolism. Cationic amino acids are transported across the placental microvillous and basal membranes by multiple pathways which act to mediate maternal/fetal transport. To identify the cationic amino acid transporters of human placenta, total RNA was harvested from cultured trophoblast and from the BeWo choriocarcinoma cell line, b30 clone, and used for reverse transcription (RT… Expand
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References

SHOWING 1-10 OF 48 REFERENCES
ASC system activity is altered by development of cell polarity in trophoblast from human placenta.
TLDR
It is concluded that membrane specialization accompanying fusion and differentiation of the cytotrophoblast to form syncytiotrophoblow results in a polarization of neutral amino acid transport systems. Expand
The amino acid transport system y+L induced in Xenopus laevis oocytes by human choriocarcinoma cell (JAR) mRNA is functionally related to the heavy chain of the 4F2 cell surface antigen.
TLDR
Results indicated that the arginine transport activity induced in Xenopus oocytes by JAR cell mRNA was due to an amino acid transport system which is identical with or highly similar to y+L and that this system was responsible for a major portion of leucine transportActivity measured in these oocytes. Expand
Expression cloning of a human renal cDNA that induces high affinity transport of L-cystine shared with dibasic amino acids in Xenopus oocytes.
TLDR
The isolation of a functional cDNA corresponding to human rBAT is shown by screening a human kidney cortex cDNA library for expression of sodium-independent transport of L-[3H]arginine in Xenopus oocytes and concludes that the isolation of this human cDNA would lead to the study of the possible involvement of rBAT in human hyperaminoacidurias. Expand
Human cationic amino acid transporters hCAT-1, hCAT-2A, and hCAT-2B: three related carriers with distinct transport properties.
TLDR
CAT-2 proteins demonstrate a higher interspecies conservation than CAT-1 proteins that are overall 86.5% identical between mouse and human and differ by seven residues in the functional domain, reflected by the functional similarity of the human carriers with their mouse homologues. Expand
Expression cloning of a cDNA from rabbit kidney cortex that induces a single transport system for cystine and dibasic and neutral amino acids.
  • J. Bertran, A. Werner, +7 authors H. Murer
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1992
TLDR
A cDNA clone is isolated by screening a rabbit kidney cortex cDNA library for expression of sodium-independent transport of L-arginine and L-alanine in Xenopus laevis oocytes and relates to a single component of Sodium- independent transport for dibasic and neutral amino acids. Expand
Molecular biology of mammalian plasma membrane amino acid transporters.
TLDR
The tissue expression, transport characteristics, structure-function relationship, and the putative physiological roles of four amino acid transporters belonging to four protein families are described. Expand
Two cationic amino acid transport systems in human placental basal plasma membranes.
TLDR
The higher Vmax of the y+ system suggests that in utero it may mediate transfer of cationic amino acids from the syncytiotrophoblast to the fetus as well as the high-affinity low-capacity bo,+ system remains to be determined. Expand
Transporters for cationic amino acids in animal cells: discovery, structure, and function.
TLDR
The roles of these transporters in nutrition, endocrinology, nitric oxide biology, and immunology, as well as in the genetic diseases cystinuria and lysinuric protein intolerance, are reviewed. Expand
Membrane potential dependence of the kinetics of cationic amino acid transport systems in human placenta.
TLDR
Analysis of the kinetic constants characterizing system y+ shows that with a change of potential from zero to negative the maximum velocity is roughly doubled and the half‐saturation constant is halved leading to a 4‐fold increase in permeability; for system y-L smaller changes are seen and Km does not change; the resulting increase in y+L permeability is 1.5‐fold. Expand
Sodium-independent lysine uptake by the BeWo choriocarcinoma cell line.
TLDR
It is concluded that like placental basal membrane, the mononuclear BeWo cell possesses two sodium-independent L-lysine transport systems, the high-capacity, NEM-sensitive, leucine-insensitive system resembles the widespread system y+. Expand
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