Identification of TH1 as an interaction partner of A-Raf kinase

@article{Yin2004IdentificationOT,
  title={Identification of TH1 as an interaction partner of A-Raf kinase},
  author={Xiang L. Yin and She. Chen and Jian Xin Gu},
  journal={Molecular and Cellular Biochemistry},
  year={2004},
  volume={231},
  pages={69-74}
}
A-Raf is an important intermediate of the growth factor Ras-MAP kinase pathway. In a two-hybrid screen of human fetal liver cDNA library, TH1 was detected as a new interaction partner of A-Raf. TH1 is a highly conserved and widely expressed protein, which was recently cloned by Bonthron DT group. The binding between A-Raf and TH1 was specific, as no binding between TH1 and B-Raf or c-Raf was observed, and the amino-terminal 162 amino acids in the A-Raf regulatory domain were found to be… 
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7 Citations
Background Citations
4
Results Citations
1

7 Citations

Trihydrophobin 1 Is a New Negative Regulator of A-Raf Kinase*
TLDR
Using confocal microscopy, it is found that TH1 colocalizes with A-Raf, which confirms its function further and provides a clue to understanding the cellular function of TH1 on Raf isoform-specific regulation.
Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis.
Trihydrophobin 1 Phosphorylation by c-Src Regulates MAPK/ERK Signaling and Cell Migration
TLDR
A novel mechanism for the comprehensive regulation of Ras/Raf/MEK/ERK signaling and cell migration involving tyrosine phosphorylation of TH1 by c-Src is demonstrated.
Expression and distribution of trihydrophobin 1 in postnatal developing mouse testis
TLDR
The human trihydrophobin 1 (TH1) is a highly conserved and widely expressed protein and it is clear that TH1 serves as a new specific negative regulator of A-Raf kinase in mouse testis by using coimmunoprecipitation analysis.
Negative role of trihydrophobin 1 in breast cancer growth and migration
TLDR
The protein level of TH1 was negatively correlated with the aggressiveness of human breast cancer, suggesting that TH1 might play an important role in regulation of proliferation and invasion in human breast cancers, and could be a potential target forhuman breast cancer treatment.
Trihydrophobin 1 attenuates androgen signal transduction through promoting androgen receptor degradation
TLDR
Results indicate that TH1 is a novel regulator to control the duration and magnitude of androgen signal transduction and might be directly involved in androgen‐related developmental, physiological, and pathological processes.
The clinical significance of small copy number variants in neurodevelopmental disorders
TLDR
These results verify the diagnostic relevance of genome-wide rare CNVs <500 kb, which were found pathogenic in ∼2% (14/714) of cases and highlight their inherent potential for discovery of new conditions.

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