Identification of Suitable Reference Genes for Normalization of Real-Time Quantitative Polymerase Chain Reaction in an Intestinal Graft-Versus-Host Disease Mouse Model.

@article{Li2015IdentificationOS,
  title={Identification of Suitable Reference Genes for Normalization of Real-Time Quantitative Polymerase Chain Reaction in an Intestinal Graft-Versus-Host Disease Mouse Model.},
  author={Xiaoqian Li and Jianlin Qiao and Na Yang and Hongling Mi and Peipei Chu and Yan Xia and Haina Yao and Yonghai Liu and Kunming Qi and Zhiling Yan and Ling-yu Zeng and Kailin Xu},
  journal={Transplantation proceedings},
  year={2015},
  volume={47 6},
  pages={2017-25}
}
BACKGROUND With the development of real-time quantitative polymerase chain reaction (RT-qPCR) and intensive research on acute graft-versus-host disease (GVHD), selecting the best reference gene for normalization of RT-qPCR analysis in a GVHD model becomes more and more important. In this study, we aimed to identify suitable reference genes for mRNA studies in an intestinal GVHD mouse model after bone marrow transplantation (BMT). METHODS BALB/c recipients received 7.5 Gy total body… CONTINUE READING
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