Identification of Suitable Reference Genes for Peripheral Blood Mononuclear Cell Subset Studies in Multiple Sclerosis.

@article{Oturai2016IdentificationOS,
  title={Identification of Suitable Reference Genes for Peripheral Blood Mononuclear Cell Subset Studies in Multiple Sclerosis.},
  author={Ditte Bang Oturai and Helle Bach S\ondergaard and Lars B{\"o}rnsen and Finn Thorup Sellebjerg and Jeppe Romme Christensen},
  journal={Scandinavian journal of immunology},
  year={2016},
  volume={83 1},
  pages={72-80}
}
Quantitative real-time PCR (qPCR) involves the need of a proper standard for normalizing the gene expression data. Different studies have shown the validity of reference genes to vary greatly depending on tissue, cell subsets and experimental context. This study aimed at the identification of suitable reference genes for qPCR studies using different peripheral blood cell subsets (whole blood (WB) cells, peripheral blood mononuclear cells (PBMCs) and PBMC subsets (CD4(+) T cells, CD8(+) T cells… CONTINUE READING
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