Identification of SCF Ubiquitin Ligase Substrates by Global Protein Stability Profiling

@article{Yen2008IdentificationOS,
  title={Identification of SCF Ubiquitin Ligase Substrates by Global Protein Stability Profiling},
  author={Hsueh-Chi S. Yen and Stephen J. Elledge},
  journal={Science},
  year={2008},
  volume={322},
  pages={923 - 929}
}
Ubiquitin-mediated proteolysis regulates all aspects of cellular function, and defects in this process are associated with human diseases. The limited number of identified ubiquitin ligase–substrate pairs is a major bottleneck in the ubiquitin field. We established and applied genetic technologies that combine global protein stability (GPS) profiling and genetic perturbation of E3 activity to screen for substrates of the Skp1–cullin–F-box (SCF) ubiquitin ligase in mammalian cells. Among the… 

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