Identification of RhoGAP22 as an Akt-dependent regulator of cell motility in response to insulin.

@article{Rowland2011IdentificationOR,
  title={Identification of RhoGAP22 as an Akt-dependent regulator of cell motility in response to insulin.},
  author={Alexander F. Rowland and Mark Larance and William E. Hughes and David E. James},
  journal={Molecular and cellular biology},
  year={2011},
  volume={31 23},
  pages={4789-800}
}
Insulin exerts many of its metabolic actions via the canonical phosphatidylinositide 3 kinase (PI3K)/Akt pathway, leading to phosphorylation and 14-3-3 binding of key metabolic targets. We previously identified a GTPase-activating protein (GAP) for Rac1 called RhoGAP22 as an insulin-responsive 14-3-3 binding protein. Insulin increased 14-3-3 binding to RhoGAP22 fourfold, and this effect was PI3K dependent. We identified two insulin-responsive 14-3-3 binding sites (pSer(16) and pSer(395)) within… CONTINUE READING