Identification of Residues Surrounding the Active Site of Type A Botulinum Neurotoxin Important for Substrate Recognition and Catalytic Activity

@article{Ahmed2008IdentificationOR,
  title={Identification of Residues Surrounding the Active Site of Type A Botulinum Neurotoxin Important for Substrate Recognition and Catalytic Activity},
  author={S-U. Ahmed and Mark Anthony Olson and Matthew L. Ludivico and Janice Gilsdorf and Leonard August Rae Smith},
  journal={The Protein Journal},
  year={2008},
  volume={27},
  pages={151-162}
}
Type A botulinum neurotoxin is one of the most lethal of the seven serotypes and is increasingly used as a therapeutic agent in neuromuscular dysfunctions. Its toxic function is related to zinc-endopeptidase activity of the N-terminal light chain (LC) on synaptosome-associated protein-25 kDa (SNAP-25) of the SNARE complex. To understand the determinants of substrate specificity and assist the development of strategies for effective inhibitors, we used site-directed mutagenesis to investigate… CONTINUE READING
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