Identification of Proteinaceous Material in the Bone of the Dinosaur Iguanodon

  title={Identification of Proteinaceous Material in the Bone of the Dinosaur Iguanodon},
  author={G. Embery and A. Milner and R. Waddington and R. Hall and M. Langley and A. Milan},
  journal={Connective Tissue Research},
  pages={41 - 46}
This study has directed attention at the search for bone-related proteins in an extract of demineralized rib bone of the 120 mya Iguanodon . The inner compact bone was demineralized and the GuCl extract resolved into 11 fractions using anion exchange chromatography, which all contained silver-reactive proteins with various amino acid profiles. Two specific fractions, iv and xi, revealed characteristics typical of contemporary phosphoproteins and proteoglycans, respectively. Fraction iv, 43-57… Expand
Empirical Evaluation of Bone Extraction Protocols
It is found that the use of hydrochloric acid, rather than ethylenediaminetetraacetic acid, for demineralization resulted in the cleanest extractions because the acid was easily removed, and both guanidine HCl and ammonium bicarbonate were effective for extracting many bone proteins, resulting in similar electrophoretic patterns. Expand
Proteome degradation in fossils: investigating the longevity of protein survival in ancient bone
Of these 'survivor proteins' A2HSG shows a remarkable amount of sequence variation, making it potentially one of the most useful proteins to study for species identification and phylogenetic inference in archaeological and palaeontological bone. Expand
Preservation of Soft Tissues in Dinosaur Fossils: Compatibility with an Age of Millions of Years
Abstract The recent discovery of preserved cells and soft tissues in certain dinosaur bones seems incompatible with an age of millions of years, given the expectation that cells and soft tissuesExpand
EVA technology and proteomics: a two-pronged attack on Cultural Heritage.
A novel way for exploring the world Cultural Heritage in the absence of damage or contamination of the items under investigation is here reported, called the EVA technique. It is based on films ofExpand
Proteomes of the past: the pursuit of proteins in paleontology
Morphological and molecular investigations show that original biochemistry is geologically extensive, geographically global, and taxonomically wide-ranging. Expand
Scanning transmission X‐ray microscopy study of microbial calcification
Calcium phosphates and calcium carbonates are among the most prevalent minerals involved in microbial fossilization. Characterization of both the organic and mineral components in biomineralizedExpand
Will amber inclusions provide the first glimpse of a Mesozoic proteome?
The isolation of DNA fragments from a Late Cretaceous dinosaur bone preserved in bituminous strata provides compelling evidence that proteins might endure geological time better, and it has been suggested that the preservation of cells, and even soft tissues, may be possible. Expand
Collagen remnants in ancient bone
Ancient bone collagen retains valuable information. Radiocarbon dating, thermal dating, species identification, cladistics analyses, and paleodietary reconstruction efforts all use bone collagen fromExpand
Glycocalyx breakdown is increased in African children with cerebral and uncomplicated falciparum malaria
  • T. Yeo, Peggy A. Bush, +7 authors J. Weinberg
  • Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2019
Endothelial GCX degradation may impair endothelial NO production, exacerbate adhesion‐molecule expression, exposure, and parasite sequestration, and contribute to malaria pathogenesis. Expand
Glycocalyx Breakdown Is Associated With Severe Disease and Fatal Outcome in Plasmodium falciparum Malaria
  • T. Yeo, J. Weinberg, +9 authors N. Anstey
  • Medicine
  • Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 2019
Increased endothelial glycocalyx breakdown is associated with severe disease and a fatal outcome in adults with falciparum malaria and inversely associated with endothelial NO bioavailability. Expand


The Isolation and Detection of Non-Collagenous Proteins from the Compact Bone of the Dinosaur Iguanodon
The isolation of guanidinium chloride extractable protein from deminer-alised bone extracts obtained from the 125–130 mya dinosaur Iguanodon represents the first electro-phoretically defined proteins isolated from dinosaur tissues and suggests the presence of proteoglycans. Expand
Proteins in the fossil bone of the dinosaur, seismosaurus
Proteins have been successfully extracted from the fossil vertebra of a 150-million-year-old sauropod dinosaur recently excavated from the Morrison Formation of New Mexico to provide useful knowledge concerning the evolution of skeletal molecules and the fossilization process. Expand
Characterization of the major non-collagenous proteins of chicken bone: identification of a novel 60 kDa non-collagenous phosphoprotein.
A novel approximately 60 kDa protein was identified with a unique N-terminal amino acid sequence and several internal amino acid sequences and it was demonstrated that the avian form of this protein is more than 80% conserved for all the peptides sequenced in this study. Expand
Preservation of the bone protein osteocalcin in dinosaurs
Preservation of OC in fossil bones appears to be strongly dependent on the burial history and not simply on age, and is a first step toward a molecular phylogeny of the dinosaurs. Expand
Preservation of biomolecules in cancellous bone of Tyrannosaurus rex
ABSTRACT An exceptionally well preserved specimen of the tyrannosaurid dinosaur Tyrannosaurus rex Osborn shows little evidence of permineralization or other diagenetic effects. It appears that theExpand
Slow rates of degradation of osteocalcin: Green light for fossil bone protein?
Our claim, published in this journal, for successful immunodetection of the protein osteocalcin in dinosaur bone has been challenged on the grounds that the findings are inconsistent with theExpand
Phosphophoryns—major noncollagenous proteins of rat incisor dentin
Two collagen phosphop protein complex peptides were isolated, demonstrating the probable direct covalent interaction of a dentin phosphoprotein with the collagen of the mineralized matrix of rat incisor dentin. Expand
Altered Phosphorylation of Rat Dentine Phosphoproteins by Fluoride In Vivo
The fluoride-induced alterations to the biochemical structure of dentine phosphoproteins would appear to influence the phosphorylation of these macromolecules only, possibly affecting posttranslational events and play a role in disrupting the patterns of mineralization seen during fluorosis. Expand
Histochemical demonstration of DNA in osteocytes from dinosaur bones.
  • R. Pawlicki
  • Chemistry, Medicine
  • Folia histochemica et cytobiologica
  • 1995
It is suggested that an intact cell sheath enabled the preservation of DNA, though it simultaneously prevented the penetration of reagents and dyes into the cells in unsectioned material, in osteocytes with porous sheaths. Expand
Multiple forms of rat dentin phosphoproteins.
The nature of the phosphoproteins in HP was further studied after total removal of the phosphate with an insoluble form of bovine intestinal alkaline phosphatase, and the dephosphorylated product (dP-HP) gave a single major band on gel electrophoresis but showed evidence for two closely related NH2-terminal sequences. Expand