Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome.

@article{Pontvianne2016IdentificationON,
  title={Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome.},
  author={Fr{\'e}d{\'e}ric Pontvianne and Marie-Christine Carpentier and Nathalie Durut and Veronika Pavli{\vs}tov{\'a} and Karin Ja{\vs}ke and {\vS}{\'a}rka Schořov{\'a} and Hugues Parrinello and Marine Rohmer and Craig S. Pikaard and Miloslava Fojtov{\'a} and Jiř{\'i} Fajkus and Julio S{\'a}ez-V{\'a}squez},
  journal={Cell reports},
  year={2016},
  volume={16 6},
  pages={1574-1587}
}
The nucleolus is the site of rRNA gene transcription, rRNA processing, and ribosome biogenesis. However, the nucleolus also plays additional roles in the cell. We isolated nucleoli using fluorescence-activated cell sorting (FACS) and identified nucleolus-associated chromatin domains (NADs) by deep sequencing, comparing wild-type plants and null mutants for the nucleolar protein NUCLEOLIN 1 (NUC1). NADs are primarily genomic regions with heterochromatic signatures and include transposable… CONTINUE READING
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