Identification of N-acetylmethionine as the product released during the NH2-terminal processing of a pseudo-class I actin.

@article{Sheff1989IdentificationON,
  title={Identification of N-acetylmethionine as the product released during the NH2-terminal processing of a pseudo-class I actin.},
  author={David R. Sheff and Peter A. Rubenstein},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 19},
  pages={
          11491-6
        }
}
Genes for the various isoactins define two classes of actin. Class I actin genes code for Met-Asp(Glu)-actin, and class II actin genes code for Met-X-Asp(Glu)-actin where X is usually cysteine. Amino termini of both are removed in an acetylation-dependent processing reaction yielding acetyl-Asp(Glu)-actin. Both classes are processed at approximately equal rate (t1/2 = 15 min) in vivo. In vitro, class II actins are 90% processed by endogenous enzymes after 60 min in a rabbit reticulocyte lysate… CONTINUE READING