Identification of MINUS, a small polypeptide that functions as a microtubule nucleation suppressor.

@article{Fanara1999IdentificationOM,
  title={Identification of MINUS, a small polypeptide that functions as a microtubule nucleation suppressor.},
  author={P Fanara and Bj{\"o}rn Oback and Keith Ashman and Alexandre V Podtelejnikov and Richard E. Brandt},
  journal={The EMBO journal},
  year={1999},
  volume={18 3},
  pages={565-77}
}
In eukaryotic cells, tubulin polymerization must be regulated precisely during cell division and differentiation. To identify new mechanisms involved in cellular microtubule formation, we isolated an activity that suppresses microtubule nucleation in vitro. The activity was due to a small acidic polypeptide of 4.7 kDa which we named MINUS (microtubule nucleation suppressor). MINUS inhibited tau- and taxol-mediated microtubule assembly in vitro and was inactivated by dephosphorylation. The… CONTINUE READING

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Immunofluorescence population assays for studying MAP function

  • P. Fanara, R. Brandt
  • 1997
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