Identification of Larval Anisakis spp. (Nematoda: Anisakidae) in Alaska Pollock (Theragra chalcogramma) in Northern Japan Using Morphological and Molecular Markers

  title={Identification of Larval Anisakis spp. (Nematoda: Anisakidae) in Alaska Pollock (Theragra chalcogramma) in Northern Japan Using Morphological and Molecular Markers},
  author={Karl Marx A. Quiazon and Tomoyoshi Yoshinaga and M D Santos and K. Ogawa},
  booktitle={Journal of Parasitology},
Abstract The Alaska pollock, Theragra chalcogramma (Pallas), is an important raw source for surimi and other food products in Japan. However, Alaska pollock caught in the Atlantic and Mediterranean regions has been reported to harbor Anisakis species that pose considerable food safety problems. Here, we identified the third-stage (L3) Anisakis spp. sampled from Alaska pollock caught in northern Japan using a combination of morphological and molecular analyses which included PCR-RFLP and… 

Global assessment of molecularly identified Anisakis Dujardin, 1845 (Nematoda: Anisakidae) in their teleost intermediate hosts.

Comparison with host records from 60 previous molecular studies of Anisakis species reveals the teleost host range so far recorded for the genus, and different intermediate host compositions implicate differing life cycles for the so far molecularly identified An Isakis sibling species.

Occurrence and Molecular Identification of Anisakis Dujardin, 1845 from Marine Fish in Southern Makassar Strait, Indonesia

The difference of 4 bp nucleotides in ITS1 regions and divergence into 2 subgroups in mtDNA cox2 indicating the existence of A. typica sibling species in the Makassar Strait is indicated.

Morphological and genetic identification of Anisakis paggiae (Nematoda: Anisakidae) in dwarf sperm whale Kogia sima from Brazilian waters.

Integrative taxonomic analysis allowed the identification of A. paggiae for the first time in Brazilian waters, providing new data about their geographical distribution and presenting the first SEM images of this species.

Population Structure of Three Species of Anisakis Nematodes Recovered From Pacific Sardines (Sardinops sagax) Distributed Throughout the California Current System

abstract:  Members of the Anisakidae are known to infect over 200 pelagic fish species and have been frequently used as biological tags to identify fish populations. Despite information on the global

Occurrence of Anisakid Nematodes in Walleye Pollock (Gadus chalcogrammus) Caught off the East Sea of Korea: Their Molecular Identification and Biological Implication

Clues to the geographical distribution, migration route and prey items of walleye pollock caught off Korea are found and mitochondrial DNA cox2 gene sequences of randomly selected A. simplex showed the highest similarity (99.5%–98.0%) with those from bearded seals in Japan.

Integrative taxonomy of anisakid nematodes in stranded cetaceans from Brazilian waters: an update on parasite’s hosts and geographical records

The results clearly indicated that the preservation of nematodes influenced significantly on the level of taxonomic definition, in both morphological and, mainly, genetic analyses.



A new species of Anisakis Dujardin, 1845 (Nematoda, Anisakidae) from beaked whales (Ziphiidae): allozyme and morphological evidence

A new Anisakis species was identified, showing fixed allele differences at a number of loci from the other Anisaki spp.

Evidence for a new species of Anisakis Dujardin, 1845: morphological description and genetic relationships between congeners (Nematoda: Anisakidae)

A low genetic identity was detected at allozyme level between the eight Anisakis species and was supported by differences in adult and larval morphology, as well as with respect to their main definitive hosts.


One-hundred and fifteen anisakid larvae from 3 different fish hosts, Aphanopus carbo, Scomber japonicus, and Trachurus picturatus, caught in Madeiran waters, were identified by PCR-RFLP and an apparently undescribed taxon seems to be more similar to A. ziphidarum than to other species of the genus.

Genetic markers in the study of Anisakis typica (Diesing, 1860): larval identification and genetic relationships with other species of Anisakis Dujardin, 1845 (Nematoda: Anisakidae)

The present findings represent the first established records of intermediate/paratenic hosts of Anisakis typica and extend its range to Somali waters of the Indian Ocean and to the Eastern Mediterranean Sea.

Allozyme and morphological identification of shape Anisakis, Contracaecum and Pseudoterranova from Japanese waters (Nematoda, Ascaridoidea)

Allozyme markers were used to identify anisakid nematodes from marine Japanese waters, morphologically assigned to three species complexes: Anisakis simplex, Contracaecum osculatum and Pseudoterranova decipiens, which was found to correspond morphologically to Porrocaecum azarasi, previously considered a synonym of P.decipiens.

Molecular systematics, phylogeny and ecology of anisakid nematodes of the genus Anisakis Dujardin, 1845: an update.

Preliminary data for reconstruction of a possible co-evolutionary scenario between cetacean hosts and their Anisakis endoparasites suggests that cospeciation and host-switching events may have accompanied the evolution of this group of parasites.

A Quantitative Study of Economically Important Parasites of Walleye Pollock (Theragra chalcogramma) from British Columbian Waters and Effects of Postmortem Handling on Their Abundance in the Musculature

The identity of parasites infecting the musculature and body cavity of walleye pollock collected from two British Columbian localities, their distribution within the host's Musculature, and the influence of host sex, length, and method of postmortem handling on parasite prevalence and abundance were determined were determined.

Molecular characterization of Anisakis pegreffii larvae in Pacific cod in Japan

The present study is the first to confirm the distribution of A. pegreffii in Japan, and to detect A. peelffii larvae in Pacific cod.