Identification of IFN-gamma-producing CD4+ T cells following PMA stimulation.

  title={Identification of IFN-gamma-producing CD4+ T cells following PMA stimulation.},
  author={K. von Kemp and Helle Bruunsgaard},
  journal={Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research},
  volume={21 7},
Treatment of T cells with phorbol esters, such as phorbol myristate acetate (PMA), induces downregulation of CD4, making unambiguous identification of this subset difficult. In this study, the kinetics of intracellular expression of interferon-gamma (IFN-gamma) and downmodulation of surface CD4 were measured in peripheral blood mononuclear cells (PBMC) after PMA stimulation. The number of IFN-gamma-producing cells increased within a 4-h period while the fluorescence intensity of the CD4(+) cell… CONTINUE READING
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