Identification of Human UGT2B7 as the Major Isoform Involved in the O-Glucuronidation of Chloramphenicol

  title={Identification of Human UGT2B7 as the Major Isoform Involved in the O-Glucuronidation of Chloramphenicol},
  author={Mei Chen and Barbara Leduc and S G Kerr and David Howe and David A R Williams},
  journal={Drug Metabolism and Disposition},
  pages={368 - 375}
Chloramphenicol (CP), a broad spectrum antibiotic, is eliminated in humans by glucuronidation. The primary UGT enzymes responsible for CP O-glucuronidation remain unidentified. We have previously identified the 3-O-CP (major) and 1-O-CP (minor) glucuronides by β-glucuronidase hydrolysis, liquid chromatography-tandem mass spectrometry, and 1D/2D H NMR. Reaction phenotyping for the glucuronidation of CP with 12 expressed human liver UGT isoforms has identified UGT2B7 as having the highest… 

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