Identification of HLA-DRB1 * 114 by oligonucleotide typing and DNA sequencing

This report describes a new DRB1 allele, identified in a caucasoid individual, which has an identical sequence to DRBI*l l02 except at the location of codon 86. The nucleotide substitution (GTG to GGT) at this codon corresponds to an amino acid substitution in the new allele. Genomic DNA was extracted from 10 gl frozen whole blood using Chelex-100 resin… CONTINUE READING