Identification of Giardia species and Giardia duodenalis assemblages by sequence analysis of the 5.8S rDNA gene and internal transcribed spacers

@article{Cacci2010IdentificationOG,
  title={Identification of Giardia species and Giardia duodenalis assemblages by sequence analysis of the 5.8S rDNA gene and internal transcribed spacers},
  author={Simone Mario Cacci{\`o} and Relja Beck and Andr{\'e} Almeida and Anna Bajer and E Pozio},
  journal={Parasitology},
  year={2010},
  volume={137},
  pages={919 - 925}
}
SUMMARY PCR assays have been developed mainly to assist investigations into the epidemiology of Giardia duodenalis, the only species in the Giardia genus having zoonotic potential. However, a reliable identification of all species is of practical importance, particularly when water samples and samples from wild animals are investigated. The aim of the present work was to genotype Giardia species and G. duodenalis assemblages using as a target the region spanning the 5.8S gene and the 2 flanking… 
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Genotyping Giardia duodenalis isolates from dogs: lessons from a multilocus sequence typing study.
Giardiasis is a common infection of dogs, and the occurrence of both zoonotic and host-adapted assemblages of Giardia duodenalis is well documented in this host. In the current study, G. duodenalis…
Prevalence and molecular typing of Giardia spp. in captive mammals at the zoo of Zagreb, Croatia.
Occurrence and genetic determination of Giardia in dogs from South Eastern Europe
TLDR
The results demonstrate that G. duodenalis should be considered as a common parasite in dogs from South Eastern Europe, however, there was no evidence for zoonotic Giardia assemblages in the investigated canine subpopulation.
Giardia microti in pet Microtus guentheri: Evidence of a parasite never detected in Italy.
Detection of zoonotic and livestock-specific assemblages of Giardia duodenalis in free-living wild lizards.
TLDR
The detection of zoonotic and livestock-specific assemblages of G. duodenalis in free-living lizards demonstrates the wide environmental contamination by this parasite, possibly due to human activities.
Multilocus sequence typing of canine Giardia duodenalis from South Eastern European countries
TLDR
The results demonstrate that G. duodenalis should be considered as a common parasite in dogs from South Eastern Europe and there was no evidence for zoonotic Giardia assemblages in the investigated canine subpopulation.
Real-Time PCR for Molecular Detection of Zoonotic and Non-Zoonotic Giardia spp. in Wild Rodents
TLDR
A workflow based on newly developed real-time PCR schemes targeting the small ribosomal RNA multi-copy gene locus to distinguish G. duodenalis genotypes (assemblages) A and B has the potential to facilitate the detection of potentially zoonotic and non-zoonotic Giardia species in wild rodents.
Zoonotic potential of Giardia.
Giardia/giardiasis - a perspective on diagnostic and analytical tools.
First Molecular Detection of Giardia duodenalis Assemblage B in a Free-Living European Wildcat (Felis s. silvestris) from Luxembourg
TLDR
This is the first detection and genotyping of G. duodenalis in a European wild felid in general, and of assemblage B in particular, recovered from faeces of free-living European wildcats from Luxembourg.
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