Identification of Flavobacterium columnare by a species-specific polymerase chain reaction and renaming of ATCC43622 strain to Flavobacterium johnsoniae.

@article{Darwish2004IdentificationOF,
  title={Identification of Flavobacterium columnare by a species-specific polymerase chain reaction and renaming of ATCC43622 strain to Flavobacterium johnsoniae.},
  author={Ahmed M. Darwish and Adnan A Ismaiel and Joseph C. Newton and Jane Tang},
  journal={Molecular and cellular probes},
  year={2004},
  volume={18 6},
  pages={421-7}
}
Species-specific polymerase chain reaction (PCR) primers have been designed to identify the causative agent of columnaris disease, Flavobacterium columnare. The 16S rRNA gene sequences of F. columnare (eight sequences representing the different genotypes of the species) and related species (18 sequences) were aligned and compared to choose specific regions that are unique to F. columnare and do not have significant intraspecies variability. The species-specific regions in the 16S rRNA gene were… CONTINUE READING

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References

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Showing 1-10 of 17 references

A simple procedure for identification of Cytophaga columnaris

  • BR Griffin
  • J Aquat Anim Health
  • 1992
Highly Influential
8 Excerpts

Flavobacterial diseases: columnaris disease, cold-water disease and bacterial gill disease

  • EB Shotts, CE Starliper
  • Fish diseases and disorders. Viral, bacterial and…
  • 1999
Highly Influential
4 Excerpts

Identification of Flavobacterium and Flexibacter species by species specific polymerase chain reaction primers to the 16S ribosomal RNA gene

  • JA Bader, EB ShottsJr.
  • J Aquat Anim Health
  • 1998
Highly Influential
7 Excerpts

Identification of Flexibacter maritimus, Flavobacterium branchiophilum and Cytophaga columnaris by PCR targeted 16S ribosomal DNA

  • T Toyama, K Kita-Tsukamoto, H. Wakabayashi
  • Fish Pathol 1996;31:25–31
  • 1996
Highly Influential
5 Excerpts

Genotyping of strains of Flavobacterium columnare from diseased fishes

  • A Triyanto, H. Wakabayashi
  • Fish Pathol
  • 1999
Highly Influential
3 Excerpts

Health maintenance and principal microbial diseases of cultured fishes

  • JA Plumb
  • Ames, IA: Iowa State University
  • 1999
Highly Influential
6 Excerpts

Short protocols in molecular biology

  • FM Ausubel, R Brent, +4 authors K. Struhl
  • 1999
Highly Influential
3 Excerpts

Rapid detection of columnaris disease in channel catfish (Ictalurus punctatus) with a new species-specific 16-S rRNA gene-based PCR primer for Flavobacterium columnare

  • JA Bader, CA Shoemaker, PH. Klessius
  • J Microbiol Methods 2003;52:209–20
  • 2003
2 Excerpts

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