Identification of Essential Filovirion-associated Host Factors by Serial Proteomic Analysis and RNAi Screen*

  title={Identification of Essential Filovirion-associated Host Factors by Serial Proteomic Analysis and RNAi Screen*},
  author={Kevin B. Spurgers and Timothy G. Alefantis and Brian D. Peyser and Gordon T Ruthel and Alison A Bergeron and Julie A Costantino and Sven Enterlein and Krishna P. Kota and Robert C. Boltz and M. Javad Aman and Vito G. DelVecchio and Sina Bavari},
  journal={Molecular \& Cellular Proteomics},
  pages={2690 - 2703}
An assessment of the total protein composition of filovirus (ebolavirus and marburgvirus) virions is currently lacking. In this study, liquid chromatography-linked tandem mass spectrometry of purified ebola and marburg virions was performed to identify associated cellular proteins. Host proteins involved in cell adhesion, cytoskeleton, cell signaling, intracellular trafficking, membrane organization, and chaperones were identified. Significant overlap exists between this data set and proteomic… 

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