Identification of Eimeria tenella genes encoding for secretory proteins and evaluation of candidates by DNA immunisation studies in chickens.

@article{Klotz2007IdentificationOE,
  title={Identification of Eimeria tenella genes encoding for secretory proteins and evaluation of candidates by DNA immunisation studies in chickens.},
  author={Christian Klotz and Florian Gehre and Richard Lucius and Thomas Pogonka},
  journal={Vaccine},
  year={2007},
  volume={25 36},
  pages={6625-34}
}
In order to identify secretory proteins as possible new vaccine candidates, a cDNA-library from E. tenella sporozoites was generated in yeast and was used to select secreted and surface proteins. Herein 191 clones were isolated and analysis of the nucleic acid sequences revealed 162 deduced open reading frames with a prediction for signal peptides. These sequences are characterized by high redundancy, comprising 25 unique protein fragments with a high degree of stage specificity. Only three… CONTINUE READING