Identification of Echinacoside Metabolites Produced by Human Intestinal Bacteria Using Ultraperformance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry.

@article{Li2015IdentificationOE,
  title={Identification of Echinacoside Metabolites Produced by Human Intestinal Bacteria Using Ultraperformance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry.},
  author={Yang-yang Li and Guisheng Zhou and Shihua Xing and Pengfei Tu and Xiaobo Li},
  journal={Journal of agricultural and food chemistry},
  year={2015},
  volume={63 30},
  pages={
          6764-71
        }
}
Echinacoside (ECH) is one of the representative phenylethanoid glycosides. It is widely present in plants and exhibits various bioactivities. However, the extremely low oral bioavailability of ECH in rats implies that ECH may go through multiple hydrolysis steps in the gastrointestinal tract prior to its absorption into the blood. Therefore, the gastrointestinal metabolites of ECH are more likely to be the bioactive components. This study established an approach combining ultraperformance… 

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References

SHOWING 1-10 OF 33 REFERENCES
In Vitro and in Vivo Metabolite Profiling of Valnemulin Using Ultraperformance Liquid Chromatography–Quadrupole/Time-of-Flight Hybrid Mass Spectrometry
Valnemulin, a semisynthetic pleuromutilin derivative related to tiamulin, is broadly used to treat bacterial diseases of animals. Despite its widespread use, metabolism in animals has not yet been
Evaluation of the Intestinal Transport of a Phenylethanoid Glycoside-Rich Extract from Cistanche deserticola across the Caco-2 Cell Monolayer Model
TLDR
Investigating the intestinal permeability of a PhG-rich extract from CD as an integrated system in the Caco-2 cell monolayer model using a bioassay system showed that PRE is primarily transported via poorly absorbed passive diffusion down a concentration gradient without efflux, which provides the pharmacokinetic basis for the clinical application of PhGs in CD.
Intestinal bacterial hydrolysis is indispensable to absorption of 18β‐glycyrrhetic acid after oral administration of glycyrrhizin in rats
TLDR
These findings indicate that orally administered glycyrrhizin is poorly absorbed from the gut, but is hydrolysed to 18β‐glycyrrhetic acid by intestinal bacteria such as E. sp.
Microbial metabolism of caffeic acid and its esters chlorogenic and caftaric acids by human faecal microbiota in vitro.
Seasonal variations in the concentrations of lipophilic compounds and phenolic acids in the roots of Echinacea purpurea and Echinacea pallida.
TLDR
The major alkamides in the roots of E. purpurea were at their lowest concentration in the middle of autumn and early winter, and the total concentration of lipophilic compounds in E. pallida showed the same pattern.
Hydroxytyrosol is the major anti-inflammatory compound in aqueous olive extracts and impairs cytokine and chemokine production in macrophages.
TLDR
The effects of HT on NO and chemokine production point to their impact on chronic inflammatory processes in endothelium or arthritis.
Role of intestinal microbiota in the generation of polyphenol-derived phenolic acid mediated attenuation of Alzheimer's disease β-amyloid oligomerization.
TLDR
Important contribution of the intestinal microbiota to the protective activities of GSPE (as well as other polyphenol preparations) in AD is suggested.
...
...