Identification of ERK2-binding domain of EBITEIN1, a novel ERK2-binding protein.

@article{Miura2008IdentificationOE,
  title={Identification of ERK2-binding domain of EBITEIN1, a novel ERK2-binding protein.},
  author={Kenji Miura and Junko Imaki},
  journal={Biochimica et biophysica acta},
  year={2008},
  volume={1784 9},
  pages={
          1319-25
        }
}
  • K. Miura, J. Imaki
  • Published 1 September 2008
  • Biology, Medicine
  • Biochimica et biophysica acta
We recently cloned a cDNA encoding a novel extracellular signal-regulated kinase 2 (ERK2) binding protein, EBITEIN1, by yeast two-hybrid screening. In this study, we further characterized EBITEIN1. Binding experiments using various deletion mutants identified a 40-amino acid minimal sequence for binding ERK2. Binding experiments using substitution mutants indicated the crucial role of arginine residues in this sequence. Based on empirical and bioinformatic analyses, we propose two domains in… Expand

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