Identification of EMS-induced causal mutations in Arabidopsis thaliana by next-generation sequencing.

@article{Uchida2014IdentificationOE,
  title={Identification of EMS-induced causal mutations in Arabidopsis thaliana by next-generation sequencing.},
  author={Naoyuki Uchida and Tomoaki Sakamoto and Masao Tasaka and Tetsuya Kurata},
  journal={Methods in molecular biology},
  year={2014},
  volume={1062},
  pages={259-70}
}
Emerging next-generation sequencing (NGS) technologies are powerful tools for the identification of causal mutations underlying phenotypes of interest in Arabidopsis thaliana. Based on a methodology termed bulked segregant analysis (BSA), whole-genome sequencing data are derived from pooled F2 segregants after crossing a mutant to a different polymorphic accession and are analyzed for single nucleotide polymorphisms (SNPs). Then, a genome region spanning the causal mutation site is narrowed… CONTINUE READING
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