Identification of Ditylenchus species associated with Fabaceae seeds based on a specific polymerase chain reaction of ribosomal DNA-ITS regions

@article{Kerkoud2006IdentificationOD,
  title={Identification of Ditylenchus species associated with Fabaceae seeds based on a specific polymerase chain reaction of ribosomal DNA-ITS regions},
  author={Mohamed Kerkoud and Magali Esquibet and Olivier Plantard and Myriam Avrillon and Corinne Guimier and Martine Franck and Jo{\"e}l L{\'e}chapp{\'e} and Ren{\'e} Mathis},
  journal={European Journal of Plant Pathology},
  year={2006},
  volume={118},
  pages={323-332}
}
A technique based on the use of specific primers for polymerase chain reaction (PCR) was developed for the identification of the stem and bulb nematode belonging to the Ditylenchus dipsaci species complex. The internal transcribed spacer region ITS1 and ITS2, the gene 5.8 S and part of genes 18 S and 26 S of twenty populations of the D. dipsaci species complex belonging to both D. dipsaci sensu stricto and Ditylenchus sp. B (corresponding to populations of giant individuals associated to Vicia… CONTINUE READING