Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)

@article{Mandolino1999IdentificationOD,
  title={Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)},
  author={G. Mandolino and A. Carboni and S. Forapani and V. Faeti and P. Ranalli},
  journal={Theoretical and Applied Genetics},
  year={1999},
  volume={98},
  pages={86-92}
}
Abstract A 400-bp RAPD marker generated by a primer of random decamer sequence has been found associated with the male sex phenotype in 14 dioecious cultivars and accessions of hemp (Cannabis sativa L.). [...] Key Result The primer OPA8 generates a set of bands, most of which polymorphic among all the individual plants tested, and 1 of which, named OPA8400, present in all male plants and absent in female plants.Expand

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References

SHOWING 1-10 OF 21 REFERENCES
Identification of sex in hop (Humulus lupulus) using molecular markers.
TLDR
Compared pools of male and female plants derived from a hop cross to identify molecular markers associated with the Y or male-specific chromosome, primers developed from the DNA sequence of this product provide a marker for rapid sex identification in crosses of hop by means of PCR. Expand
A male-associated DNA sequence in a dioecious plant, Cannabis sativa L.
TLDR
Male-associated DNA sequences were analyzed in a dioecious plant, Cannabis sativa L. (family: Moraceae), which is known to have sex chromosomes and exhibited no significant similarity to previously reported sequences. Expand
Genetic diversity of Cannabis sativa germplasm based on RAPD markers
TLDR
RAPD markers provide a powerful tool for the investigation of genetic variation in cultivars/accessions of hemp and Variability within the two cultivars ‘Carmagnola’ and ‘Fibranova1’ was shown, suggesting good possibilities for long–term selection work. Expand
Genetics of sex determination in flowering plants
TLDR
Knowing of the genes that control floral organ identity in a broad range of angiosperms has now allowed detailed comparative expression studies, and sex-related differences indicate that the mechanism of sex determination is not the same in all species. Expand
Repeated DNA and heterochromatin structure in Rumex acetosa
TLDR
Seven clones, six of which represent moderate to highly repeated sequences, were isolated from a plasmid clone bank of EcoRI-digested Rumex acetosa DNA and studies of RNA transcription by these sequences indicate that one, pRa141, may be actively transcribed. Expand
Sex reversal in the female plants ofCannabis sativa by cobalt ion
TLDR
Apical application of cobalt chloride to female plants of cannabis sativa caused drying of the shoot tip and formation of axillary branches, and pollen in the flowers of altered sex were viable. Expand
Molecular Cloning: A Laboratory Manual
TLDR
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Expand
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
TLDR
A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments. Expand
Basic local alignment search tool.
A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP)Expand
A simple and rapid DNA microextraction method for plant, animal, and insect suitable for RAPD and other PCR analyses.
...
1
2
3
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