Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)

  title={Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)},
  author={Giuseppe Mandolino and Andrea Carboni and S. Forapani and Valerio Faeti and Paolo Ranalli},
  journal={Theoretical and Applied Genetics},
Abstract A 400-bp RAPD marker generated by a primer of random decamer sequence has been found associated with the male sex phenotype in 14 dioecious cultivars and accessions of hemp (Cannabis sativa L.). [] Key Result The primer OPA8 generates a set of bands, most of which polymorphic among all the individual plants tested, and 1 of which, named OPA8400, present in all male plants and absent in female plants.

Determination of a sex-related RAPD marker in Carob (Ceratonia siliqua L.)

This is the first report in the literature that one RAPD marker related to 80% reliability to male sex in carob was determined, and it was observed in the male parent and four out of five male F1 hybrids.

RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L.

The gel-blot analysis and fluorescence in situ hybridization results presented here support the hypothesis that accumulation of retrotransposable elements on the Y chromosome might be 1 cause of heteromorphism of sex chromosomes.

RAPD markers for identification of sex in pointed gourd (Trichosanthes dioica Roxb.)

The two RAPD markers, the malespecific OPC05 1000 and the female-specific marker OPC14 400 together can reliably differentiate male and female plants of T. dioica long before the plants attain maturity and flowering.

Occurrence and frequency of putatively Y chromosome linked DNA markers in Cannabis sativa L.

The fact that the association between these markers and the staminate phenotype is found when examining male plants of distantly related varieties, and that such linkage is never broken when different progenies are examined, strongly supports the hypothesis that the markers found are physically located on the Y chromosome, in a region excluded from recombination during meiosis.

Sex-specific SCAR markers in the dioecious plant Rumex nivalis (Polygonaceae) and implications for the evolution of sex chromosomes

SCAR primers based on isolated and sequenced male-specific fragments as identified in an AFLP analysis of the dioecious plant Rumex nivalis resulted in fragments exclusively present in males, in accordance with proposed modes of the evolution of sex chromosomes.

Identification of a male-specific AFLP marker in a functionally dioecious fig, Ficus fulva Reinw. ex Bl. (Moraceae)

A male-specific amplified fragment length polymorphism (AFLP) marker was identified in the functionally dioecious fig species, Ficus fulva, and sequenced and converted into a sequence characterised amplified region (SCAR) marker, suggesting that sequence divergence between male- and female-specific chromosomal regions is low.

Development of a RAPD-based SCAR marker for sex identification in Momordica dioica Roxb

Since there are no morphological markers to identify sex, an easy, rapid and reliable molecular method for sex identification at the pre-flowering stage in M. dioica is reported in this paper.

Development and application of SCAR markers for sex identification in the dioecious species Ginkgo biloba L.

The SCAR primers developed in this paper can be used as effective, convenient and reliable molecular markers for sex identification in G. biloba.

Development of male-specific SCAR marker in date palm (Phoenix dactylifera L.)

To develop such an assay, genomic DNA from 45 individual plants belonging to different varieties of date palm was subjected to PCR amplification using 100 random amplified polymorphic DNA (RAPD) and 104 intersimple sequence repeat (ISSR) primers.

Detection of sequence characterized amplified region (SCAR) markers linked to sex expression in Carica papaya L.

The Southern blot hybridization of RAPD-PCR products obtained by amplification of female, male and hermaphrodite papaya DNA amplified by OPC09 primer using radio labeled recombinant plasmid detected a polymorphic fragment in male andHermaphroditic papaya sex types.



Identification of sex in hop (Humulus lupulus) using molecular markers.

Compared pools of male and female plants derived from a hop cross to identify molecular markers associated with the Y or male-specific chromosome, primers developed from the DNA sequence of this product provide a marker for rapid sex identification in crosses of hop by means of PCR.

A male-associated DNA sequence in a dioecious plant, Cannabis sativa L.

Male-associated DNA sequences were analyzed in a dioecious plant, Cannabis sativa L. (family: Moraceae), which is known to have sex chromosomes and exhibited no significant similarity to previously reported sequences.

Genetic diversity of Cannabis sativa germplasm based on RAPD markers

RAPD markers provide a powerful tool for the investigation of genetic variation in cultivars/accessions of hemp and Variability within the two cultivars ‘Carmagnola’ and ‘Fibranova1’ was shown, suggesting good possibilities for long–term selection work.

Genetics of sex determination in flowering plants

Repeated DNA and heterochromatin structure in Rumex acetosa

Seven clones, six of which represent moderate to highly repeated sequences, were isolated from a plasmid clone bank of EcoRI-digested Rumex acetosa DNA and studies of RNA transcription by these sequences indicate that one, pRa141, may be actively transcribed.

Sex reversal in the female plants ofCannabis sativa by cobalt ion

  • H. Y. RamR. Sett
  • Biology, Chemistry
    Proceedings / Indian Academy of Sciences
  • 1979
Apical application of cobalt chloride to female plants of cannabis sativa caused drying of the shoot tip and formation of axillary branches, and pollen in the flowers of altered sex were viable.

Molecular Cloning: A Laboratory Manual

The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Basic local alignment search tool.

Sexual determination and sexual differentiation