Identification of Cell Surface Polypeptides of the Hypotrich Ciliate Euplotes octocarinatus

@article{Freiburg1993IdentificationOC,
  title={Identification of Cell Surface Polypeptides of the Hypotrich Ciliate Euplotes octocarinatus},
  author={Manfred Freiburg},
  journal={Archiv f{\"u}r Protistenkunde},
  year={1993},
  volume={143},
  pages={311-318}
}
  • M. Freiburg
  • Published 1 June 1993
  • Biology
  • Archiv für Protistenkunde
Identification and characterization of a pheromone 2 specific binding protein of Euplotes octocarinatus
TLDR
Heterologously-expressed pheromone 2 (Phr2) is used in affinity chromatography to isolate phermone 2 binding proteins in Euplotes octocarinatus and preliminary results indicate that antibodies raised against a C-terminal peptide of Phr2B inhibit cell mating.
The hypotrichous ciliate Euplotes octocarinatus has only one type of tRNACys with GCA anticodon encoded on a single macronuclear DNA molecule.
TLDR
A number of aspects which might contribute to the finding that a near-cognate tRNA isoacceptor efficiently translates the UGA stop codon are discussed, including a G:A mispairing in the first anticodon position.
UAA and UAG may Encode Amino Acid in Cathepsin B Gene of Euplotes octocarinatus
TLDR
Comparison of the derived amino acid sequence with the homologs in other eukaryotes revealed that UAA and UAG may code for glutamine in Eo‐CTSB, implying an evolutionary complexity of stop codon reassignment in eukARYotes.
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References

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TLDR
Using SDS-PAGE and immunolocalization procedures, major subunit proteins of both the plates and basal body cages of Euplotes eurystomus cells are identified.
Identification of Tetrahymena ciliary surface proteins labeled with sulfosuccinimidyl 6-(biotinamido) hexanoate and Concanavalin A and fractionated with Triton X-114.
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Tetrahymena thermophila cells were labeled with sulfosuccinimidyl 6-(biotinamido) hexanoate, a sensitive nonradioactive probe for cell surface proteins, and Western blots of axonemes and ciliary
Gamone Production in Large-Scale Cultures of Euplotes octocarinatus1
TLDR
Frequent exchange of culture fluid proved more effective than aeration in obtaining high cell densities and reasonable doubling times in large-scale cultures of the freshwater ciliate Euplotes octocarinatus.
The isolation of gamones 3 and 4 of Euplotes octocarinatus.
TLDR
Gamones 3 and 4 of the ciliate Euplotes octocarinatus were isolated and purified to chromatographic and electrophoretic homogeneity to induce cells of certain mating types to unite in pairs and exchange gametic nuclei.
Identification and Properties of Plateins, Major Proteins in the Cortical Alveolar Plates of Euplotes1
TLDR
A number of monoclonal antibodies directed against components of the cortex of Euplotes aediculatus are produced, and the name platein is proposed for a family of proteins that likely represent the major proteins comprising the alveolar plates of E. aedICulatus.
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TLDR
A comparative description of the ultrastructural features of the ciliate cell surface relevant to chemoreception, examines the structural features of putative chemoreceptive cilia, and provides a summary of the electron microscopic information available so far bearing on chemoreceptor aspects of swimming, feeding, excretion, endocytosis, and sexual responses of ciliates.
Genetic Variants of Plateins (Alveolar Plate Proteins) Among and Within Species of Euplotes1
TLDR
Intraspecific platein variants were found within the E. woodruffi complex and among strains of different geographic origin in E. aediculatus.
Mating types and mating inducing substances in Euplotes octocarinatus
TLDR
It is suggested that the transformation of the cells into cells ready for conjugation is triggered by the binding of gamone molecules to gamone-specific receptors and that mature cells usually expose receptors for all gamones except the ones they produce themselves.
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