Identification of Catalytic Amino Acid Residues by Chemical Modification in Dextranase.

Abstract

A novel endodextranase isolated from Paenibacillus sp. was found to produce isomaltotetraose and small amounts of cycloisomaltooligosaccharides with a degree of polymerization of 7-14 from dextran. To determine the active site, the enzyme was modified with 1-ethyl-3-[3- (dimethylamino)-propyl]-carbodiimide (EDC) and α-epoxyalkyl α-glucosides (EAGs), an… (More)
DOI: 10.4014/jmb.1601.01014

Topics

  • Presentations referencing similar topics