Identification of Brazilian flaviviruses by a simplified reverse transcription-polymerase chain reaction method using Flavivirus universal primers.

@article{Figueiredo1998IdentificationOB,
  title={Identification of Brazilian flaviviruses by a simplified reverse transcription-polymerase chain reaction method using Flavivirus universal primers.},
  author={Luiz T.M. Figueiredo and Weber Cheli Batista and Simone Kashima and Edamil Nassar},
  journal={The American journal of tropical medicine and hygiene},
  year={1998},
  volume={59 3},
  pages={
          357-62
        }
}
We report a simplified reverse transcription-polymerase chain reaction (RT-PCR) method for identification of Brazilian flaviviruses based on the patterns of electrophoretic separation of the amplicons. The RT-PCR was done on the culture fluids of Aedes albopictus C6/36 cells infected with Brazilian flaviviruses, without previous extraction of viral RNA, using Flavivirus universal primers that anneal to highly conserved sequences within the nonstructural protein 5 and 3'- non translated region… CONTINUE READING

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