Identification of Brachyspira hyodysenteriae-specific DNA fragments using representational difference analysis.

@article{Rothkamp2002IdentificationOB,
  title={Identification of Brachyspira hyodysenteriae-specific DNA fragments using representational difference analysis.},
  author={Anja Rothkamp and Birgit Strommenger and Gerald-F Gerlach},
  journal={FEMS microbiology letters},
  year={2002},
  volume={210 2},
  pages={
          173-9
        }
}
Two novel Brachyspira hyodysenteriae-specific DNA fragments, designated as Bh100 and Bh400, were identified using representational difference analysis. To isolate the fragments the combined DNA of the Brachyspira pilosicoli, Brachyspira intermedia, Brachyspira murdochii and Brachyspira innocens reference strains was subtracted from the genome of B. hyodysenteriae strain B204. Both fragments were present in a single copy and mapped to different positions on the genome of B. hyodysenteriae B78(T… CONTINUE READING