Identification of Bacterial Pathogens in Biofilms of Recirculating Aquaculture Systems

  title={Identification of Bacterial Pathogens in Biofilms of Recirculating Aquaculture Systems},
  author={Robin K. King and George J. Flick and Dorothy E. Pierson and Stephen A. Smith and Gregory D. Boardman and Charles W. Coale},
  journal={Journal of Aquatic Food Product Technology},
  pages={125 - 133}
  • R. King, G. Flick, C. Coale
  • Published 3 May 2004
  • Biology, Engineering
  • Journal of Aquatic Food Product Technology
Abstract Biofilms are capable of forming on all aquaculture system components, incorporating the various microflora present in the water. Pathogenic bacteria released from the biofilms are potentially capable of causing recurring diseases. Seven recirculating freshwater and two recirculating saltwater facilities were sampled, with eight different types of materials tested. Pathogenic bacteria were identified using standard bacteriological methods and commercial kits. The most significant human… 
Comparison of Bacterial Presence in Biofilms on Different Materials Commonly Found in Recirculating Aquaculture Systems
It is demonstrated that bacteria can become incorporated into a biofilm with the potential for pathogen colonization of biofilms in aquaculture systems.
Biofilms in fish processing.
This chapter discusses the three product groups, mollusk, crustacean and finfish, with regard to possible biofilm formation and methods of preventing and cleaning/disinfecting to remove the biofilm, which possibly harbors pathogens.
Response of Bacterial Biofilms in Recirculating Aquaculture Systems to Various Sanitizers
Sodium hypochlorite and peracetic acid were the most effective sanitizers, with an overall percentage reduction of GFP E. coli of approximately 2 logs10, and the quaternary ammonium compound was moderately effective against the target organisms.
The biofilm in this particular system is of little risk to stock and may even have a shielding effect through trophic processes that create equilibrium in community make-up, which may have had a protective effect against pathogenic proliferation through the mechanism of competitive exclusion.
Biofilm Formation by the Fish Pathogen Flavobacterium columnare: Development and Parameters Affecting Surface Attachment
The virulence potential of biofilm was confirmed by cutaneous inoculation of channel catfish fingerlings with mature biofilm, and several physicochemical parameters modulate attachment to surfaces, with the largest influence being exerted by hardness, salinity, and the presence of mannose.
Effects of seawater ozonation on biofilm development in aquaculture tanks.
Biofilm Formation on Aquaculture Substrates by Selected Bacterial Fish Pathogens.
  • W. Cai, C. Arias
  • Biology, Engineering
    Journal of aquatic animal health
  • 2017
It is demonstrated that common bacterial pathogens had the potential of colonizing surfaces and may use biofilm as reservoirs in fish farms and calcium had little to no effect on Edwardsiella biofilm formation.
Biofilm and Sediment are Major Reservoirs of Virulent Aeromonas hydrophila (vAh) in Catfish Production Ponds.
Over time, the concentration of Aeromonas in water samples decreased by one order of magnitude, while there was a significant increase in sediments as temperature dropped, suggesting that both environments serve as the major reservoir for this pathogen.


Biofilms in food processing environments.
  • A. C. Wong
  • Chemistry, Medicine
    Journal of dairy science
  • 1998
It is important to study the interactions between bacteria and the surfaces in a specific food processing environment to provide more effective measures for prevention of biofilm formation and for its removal.
Biofilm Development and Sanitizer Inactivation of Listeria monocytogenes and Salmonella typhimurium on Stainless Steel and Buna-n Rubber.
Scanning electron microscopy demonstrated that biofilm cells and extracellular matrices could remain on sanitized biofilms and ext racellular matrix could remain surfaces from which no viable cells were recovered.
The dynamics of biofilms
Evidence of a dormant but infective state of the fish pathogen Pasteurella piscicida in seawater and sediment
Although the biochemical, physiological, and serological characteristics; lipopolysaccharides; membrane proteins; and plasmid content of P. piscicida strains were unaffected during the starvation conditions, the dormant cells were smaller (dwarf cells) and had increased surface hydrophobicity.
Biofilm Formation by Listeria monocytogenes Utilizes a Primary Colonizing Microorganism in Flowing Systems.
Results suggest that under flowing conditions the presence of an exopolymer-producing microorganism may be more important than hydrophobicity, surface charge, or flagellar movement in attachment of L. monocytogenes to inert surfaces.
Physiological responses of bacteria in biofilms to disinfection
In situ enumeration methods using fluorescent probes and a radioisotope labelling technique were applied to evaluate physiological changes of Klebsiella pneumoniae within biofilms after disinfection treatment, showing a range of responses and suggested different physiological reactions inBiofilms exposed to chlorine and monochloramine.
The relationship between pipe material and biofilm formation in a laboratory model system
MDPE and uPVC support the lowest numbers of bacteria in a steady state biofilm in the short term (21 d) and over a longer term (7 months), and the diversity of heterotrophic bacteria was greatest on cast iron.
Use of alternative disinfectants, individually and in combination, in aquacultural wastewater treatment
The inactivation effect of chlorine, iodine, ozone and UV irradiation was compared between phosphate buffered saline and waste water collected from a fish farm, with the fish-pathogenic bacterium
Influence of hydrodynamics and nutrients on biofilm structure
Hydrodynamic conditions control two interlinked parameters; mass transfer and drag, and will, therefore, significantly influence many of the processes involved in biofilm development. The goal of
Effect of environmental stress on the ability of Listeria monocytogenes Scott A to attach to food contact surfaces.
Growth pH was also found to affect the levels of adhered cell populations to Buna-N rubber, and levels of attached cells were lower when attachment occurred under alkaline conditions.