Identification of Arabidopsis salt and osmotic stress responsive proteins using two-dimensional difference gel electrophoresis and mass spectrometry.

@article{Ndimba2005IdentificationOA,
  title={Identification of Arabidopsis salt and osmotic stress responsive proteins using two-dimensional difference gel electrophoresis and mass spectrometry.},
  author={Bongani Kaiser Ndimba and Stephen Chivasa and William J. Simon and Antoni R. Slabas},
  journal={Proteomics},
  year={2005},
  volume={5 16},
  pages={4185-96}
}
Arabidopsis thaliana cell suspension cultures have been used to investigate the effects of salinity and hyperosmotic stress on plant cellular proteins. We show that 200 mM NaCl and 400 mM sorbitol treatments induce extracellular medium acidification in Arabidopsis cell cultures, a typical response of plant cells to salt and hyperosmotic stress. Using (35)S-labelled amino acids, we demonstrated that NaCl causes a transient suppression of de novo protein synthesis, from which the cells recover… CONTINUE READING
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