Identification of Actinobacillus actinomycetemcomitans: polymerase chain reaction amplification of lktA-specific sequences.

@article{Goncharoff1993IdentificationOA,
  title={Identification of Actinobacillus actinomycetemcomitans: polymerase chain reaction amplification of lktA-specific sequences.},
  author={P Goncharoff and David H. Figurski and Roy H. Stevens and Daniel H Fine},
  journal={Oral microbiology and immunology},
  year={1993},
  volume={8 2},
  pages={105-10}
}
Actinobacillus actinomycetemcomitans has been strongly implicated in the etiology of localized juvenile periodontitis. Techniques used in the identification of this periodontal pathogen include cultural, biochemical, immunological and DNA hybridization analysis. In this study, we report the use of polymerase chain reaction (PCR) to amplify unique sequences of A. actinomycetemcomitans. Specific oligonucleotide primers LKT2 and LKT3 were designed to hybridize to the A. actinomycetemcomitans lktA… CONTINUE READING

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