Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii

@article{Makita2006IdentificationO2,
  title={Identification of 2-(Cysteinyl)amido-2-deoxy-D-galacturonic Acid Residue from the Sheath of Leptothrix cholodnii},
  author={Hiroko Makita and Yuichi Nakahara and H Fukui and Yohei Miyanoiri and Masato Katahira and Hiroko Seki and Minoru Takeda and Jun‐ichi Koizumi},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2006},
  volume={70},
  pages={1265 - 1268}
}
The sheath of Leptothrix cholodnii is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was S-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass… Expand
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