Identification of Acepromazine and Its Metabolites in Horse Plasma and Urine by LC–MS/MS and Accurate Mass Measurement

@article{Wieder2012IdentificationOA,
  title={Identification of Acepromazine and Its Metabolites in Horse Plasma and Urine by LC–MS/MS and Accurate Mass Measurement},
  author={Marcus Wieder and Bobby P Gray and Pamela Brown and Simon Hudson and Clive M. Pearce and Stuart W Paine and L L Hillyer},
  journal={Chromatographia},
  year={2012},
  volume={75},
  pages={635-643}
}
Acepromazine maleate (Sedalin®) was administered orally to six thoroughbred horses at a dose of 0.15 mg kg−1. Urine and blood samples were collected up to 412 h post-administration. Plasma and urine were hydrolysed; plasma samples were then processed using liquid–liquid extraction and urine samples using solid-phase extraction. A sensitive tandem mass spectrometric method was developed in this study, achieving a lower limit of quantification for acepromazine of 10 pg mL−1 in plasma and 100 pg… 
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