Corpus ID: 89731583

Identification andcharacterization ofacis-acting element that interferes withglucocorticoid-i

  title={Identification andcharacterization ofacis-acting element that interferes withglucocorticoid-i},
  author={H. Tanaka and A. Gustafsson},
  • H. Tanaka, A. Gustafsson
  • Published 1991
  • Biology
  • Therathepatoma cell line Ml.19isstably infected bythemousemammarytumor virus (MMTV), andthe expression ofthevirus isinduced byglucocorticoid treatment. However, inthe6.10.2 variant ofM1.19, anincrease inMMTV transcription ishardly detectable uponexposure tohormone. Themechanism ofhormone-unresponsiveness inthese cells has beenunclear. Inthis study, weshowthat nuclear extract from 6.10.2 cells contains a specific DNA-binding activity that recognizes asequence motif extending frompositions -163to… CONTINUE READING