Identification and typing of Francisella tularensis with a highly automated genotyping assay.

  title={Identification and typing of Francisella tularensis with a highly automated genotyping assay.},
  author={David D. Duncan and Amy J. Vogler and Mark J. Wolcott and Feng Li and Derek S Sarovich and D N Birdsell and Lyn M. Watson and Thomas A. Hall and Ranga Sampath and Roberta Housley and Larry B Blyn and Steve A Hofstadler and David J. Ecker and Paul S Keim and David M. Wagner and Mark W. Eshoo},
  journal={Letters in applied microbiology},
  volume={56 2},
A PCR assay was developed to genotypically characterize Francisella tularensis and F. novicida. An integrated and partially redundant set of markers was selected to provide positive identification of these species, identify subspecies of F. tularensis and genotype 14 variable number tandem repeat (VNTR) markers. Assay performance was evaluated with 117 Francisella samples. Sample DNA was amplified, and the masses of the PCR products were determined with electrospray ionization/time of flight… CONTINUE READING


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