Identification and transfer of a new cytoplasmic male sterility source from Oryza perennis into indica rice (O. sativa)

  title={Identification and transfer of a new cytoplasmic male sterility source from Oryza perennis into indica rice (O. sativa)},
  author={R. D. Dalmacio and Darshan Singh Brar and Takashige Ishii and Lesley A. Sitch and Sant S. Virmani and Gurdev S Khush},
SummaryMost of the commercial hybrids of indica rice are based on wild abortive (WA) source of cytoplasmic-genetic male sterility (CMS). Such cytoplasmic uniformity may lead to genetic vulnerability to disease and insect pests. To overcome this problem, diversification of CMS sources is essential. Crosses of 46 accessions of O. perennis and two accessions of O. rufipogon as female parents were made with two restorers (IR54, IR64) of WA cytosterility. Sterile hybrids were backcrossed with the… Expand

Figures and Tables from this paper

Genetically Characterizing a New Indica Cytoplasmic Male Sterility with Oryza glaberrima Cytoplasm for Its Potential Use in Hybrid Rice Production
The new AF CMS system should be of great potential use in hybrid rice production and confirmed the introgression of a major restorer gene from O. glaberrima into the O. sativa genome. Expand
Development of monosomic alien addition lines from the wild rice (Oryzabrachyantha A. Chev. et Roehr.) for introgression of yellow stem borer (Scirpophaga incertulas Walker.) resistance into cultivated rice (Oryzasativa L.)
Oryza brachyantha, the African wild rice which is resistant to yellow stem borer (YSB), was exploited as a source for introgression of YSB resistance trait into cultivated rice (O. sativa), through development of monosomic alien addition lines (MAALs), which were found to be eliminated in the MAALs. Expand
Development of Novel Cytoplasmic Male Sterile Source from Dongxiang Wild Rice (Oryza rufipogon)
Observation under optical microscope manifested that this novel CMS belonged to typical abortion type with less pollen compared with wild abortive type cytoplasm (CMS-WA), and inheritance analysis showed that the fertility restoration of this CMS was governed by three pairs of independent dominant genes. Expand
Evaluation of cultivars and land races of Oryza sativa for restoring and maintaining wild abortive cytoplasm
Three cytoplasmic male sterile (CMS) lines, eight improved cultivars and six landraces of rice were studied for their fertility restoring and sterility maintaining abilities and there was no strong evidence for a relationship between pollen fertility and spikelet fertility. Expand
Development of New Type of CMS Source from Dongxiang Wild Rice (Oryza rufipogon)
Observation under optical microscope manifested that this novel CMS belongs to typical abortion type with fewer number of pollens compared with CMS-WA, and inheritance analysis showed that the fertility restoration showed that it was governed by three pairs of independent dominant genes, which is the first report that fertility restoration in CMS rice is controlled by three paired dominant genes. Expand
Cytoplasmic-Nuclear Male Sterility: Origin, Evaluation and Utilization
Approaches to estimate the diversity of CMS systems to enhance the exploitation of outstanding inbred lines and also to overcome the risk of disease and pest epidemics, include both traditional field evaluation of hybrids for differential patterns of male fertility restoration and RFLP analysis of mitochondrial DNA. Expand
Gametophytically alloplasmic CMS line of rice (Oryza sativa L.) with variant orfH79 haplotype corresponds to specific fertility restorer
Sequence analysis revealed that there were a total of nine nucleotide substitutions resulting in the change of nine amino acids in the newly identified orfH79 in wild rice, which further fell into seven haplotypes, suggesting that each orFH79 haplotye represents a new CMS type and corresponds to their specific Rf allele. Expand
Characterization and Use of Male Sterility in Hybrid Rice Breeding
Attention is still urgently needed to resolve the molecular complexity of male sterility to assist rice breeding. Expand
Introgression and characterization of alloplasmic male sterile lines in brinjal (Solanum melongena L.)
High genotypic co-efficient of variability, heritability and genetic advance for all the fruit characteristics in all the generations was revealed and the availability of male sterile lines in diverse genetic backgrounds would be useful in heterosis breeding of brinjal. Expand
Methodology for creating alloplasmic soybean lines by using Glycine tomentella as a maternal parent.
This is the first report of the successful development of new alloplasmic soybean lines with cytoplasm from G. tomentella using ‘Dwight’ as the male parent. Expand


A case of cytoplasmic male sterility controlled by a restoration gene Rf was found in cultivated rice, Oryza sativa L. sativa, and the restoration gene was found to be of gametophytic type. Expand
Cytoplasmic Susceptibility in Plant Disease
A review of selected reports on disease reaction and a discussion of possible mechanisms involved in cytoplasmic suscepti­ bility in plant disease are discussed. Expand
Comparative study on the chloroplast, mitochondrial and nuclear genome differentiation in two cultivated rice species, Oryza sativa and O. glaberrima, by RFLP analyses
Cluster analyses on ct and mt DNA variations revealed that chloroplast and mitochondrial genomes were conservative within a taxon and that their differentiations were well-paralleled with respect to each other. Expand
Current Status and Future Prospects for Breeding Hybrid Rice and Wheat
This chapter reviews the present status and future prospects for breeding hybrid rice and wheat to develop varieties capable of responding to improved management and to reduce production hazards through the incorporation of genes resistant to major biological, chemical, and physical stresses. Expand
Mitochondrial DNA polymorphism in male-sterile cytoplasm of rice
The results indicate that there are a variety of mtDNAs in cytoplasmically male-sterile rice, and restriction fragment length polymorphism was observed among themtDNAs analysed. Expand
Plant Mitochondrial Genomes: Organization, Expression and Variation
RibosomalRNAs, Transfer RNAs, and Ribosomal Proteins: Causes, Ecology, and Applications. Expand
Advantages of and constraints to the use of hybrid rice varieties
  • In : K .J . Wilson (Ed
  • 1993
Properties of the circular plasmid-like DNA from mitochondria of cytoplasmic male-sterile
  • 1987