Identification and targeted disruption of the gene encoding the main 3-ketosteroid dehydrogenase in Mycobacterium smegmatis.

@article{Brzostek2005IdentificationAT,
  title={Identification and targeted disruption of the gene encoding the main 3-ketosteroid dehydrogenase in Mycobacterium smegmatis.},
  author={Anna M. Brzostek and Tomasz Sliwiński and Anna Rumijowska-Galewicz and Malgorzata Korycka-Machała and Jarosław Dziadek},
  journal={Microbiology},
  year={2005},
  volume={151 Pt 7},
  pages={2393-402}
}
The catabolic potential for sterol degradation of fast-growing mycobacteria is well known. However, no genes or enzymes responsible for the steroid degradation process have been identified as yet in these species. One of the key enzymes required for degradation of the steroid ring structure is 3-ketosteroid Delta(1)-dehydrogenase (KsdD). The recent annotation of the Mycobacterium smegmatis genome (TIGR database) revealed six KsdD homologues. Targeted disruption of the MSMEG5898 (ksdD-1) gene… CONTINUE READING

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