Identification and sequencing of chimpanzee BACs


We screened high-density filters from the RPCI-43 male chimpanzee BAC library (BACPAC resources) using hybridization probes designed to detect sequences (1) near the inner boundaries of palindromes P1–P6 and P8; (2) near P7; and (3) from a nonampliconic region of the human MSY. STS content and BAC-end sequences confirmed that, among the candidate BACs identified by hybridization, six contained the central portions of orthologues to human MSY palindromes. The BACs were sequenced as previously described. Supplementary Table 4 provides descriptions of the sequenced BACs and their GenBank accession numbers.

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@inproceedings{Sakai2003IdentificationAS, title={Identification and sequencing of chimpanzee BACs}, author={Takayoshi Sakai and Melinda Larsen and Kenneth M Yamada}, year={2003} }