Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator.

@article{Kreuzer1989IdentificationAS,
  title={Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator.},
  author={Paul Erich Kreuzer and Dagmar G{\"a}rtner and Rudolf Allmansberger and W. Hillen},
  journal={Journal of bacteriology},
  year={1989},
  volume={171 7},
  pages={
          3840-5
        }
}
The xyl operator of Bacillus subtilis W23 was identified by deletion analysis of the xyl regulatory region as a 25-base-pair (bp) sequence located 10 bp downstream from the xyl promoter. The outer 10 bp of the xyl operator exhibit perfect palindromic symmetry, while 5 central bp are nonpalindromic. It was demonstrated that the penultimate base pair near the end of this sequence is important for repressor binding. The location of the xylR gene encoding the repressor was determined by its ability… CONTINUE READING

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