Identification and reconstitution of a Na+/K+/Cl- cotransporter and K+ channel from luminal membranes of renal red outer medulla.

@article{Burnham1985IdentificationAR,
  title={Identification and reconstitution of a Na+/K+/Cl- cotransporter and K+ channel from luminal membranes of renal red outer medulla.},
  author={Catherine Burnham and Steven J D Karlish and Peter Leth J\orgensen},
  journal={Biochimica et biophysica acta},
  year={1985},
  volume={821 3},
  pages={461-9}
}
Electrophysiological studies on renal thick ascending limb segments indicate the involvement of a luminal Na+/K+/Cl- cotransport system and a K+ channel in transepithelial salt transport. Sodium reabsorption across this segment is blocked by the diuretics furosemide and bumetanide. The object of our study has been to identify in intact membranes and reconstitute into phospholipid vesicles the Na+/K+/Cl- cotransporter and K+ channel, as an essential first step towards purification of the… CONTINUE READING

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