Identification and quantitation of species in complex DNA mixtures by real-time polymerase chain reaction.

@article{LpezAndreo2005IdentificationAQ,
  title={Identification and quantitation of species in complex DNA mixtures by real-time polymerase chain reaction.},
  author={Mar{\'i}a L{\'o}pez-Andreo and Laura Lugo and Amando Garrido-Pertierra and M. I. Prieto and A. Puyet},
  journal={Analytical biochemistry},
  year={2005},
  volume={339 1},
  pages={73-82}
}
Six TaqMan real-time polymerase chain reaction (PCR) systems using minor groove binding (MGB) probes have been developed for the detection quantitation of bovine, porcine, lamb, chicken, turkey, and ostrich DNA in complex samples. Species-specific amplification was achieved by combining only two fluorogenic probes and 10 oligonucleotide primers targeting mitochondrial sequences, decreasing the cost of the assay significantly. The limits of detection ranged from 0.03 to 0.80 pg of template DNA… CONTINUE READING
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