Identification and quantification of peptides and proteins secreted from prostate epithelial cells by unbiased liquid chromatography tandem mass spectrometry using goodness of fit and analysis of variance.

@article{Florentinus2012IdentificationAQ,
  title={Identification and quantification of peptides and proteins secreted from prostate epithelial cells by unbiased liquid chromatography tandem mass spectrometry using goodness of fit and analysis of variance.},
  author={Angelica K. Florentinus and P. Bowden and Girish Sardana and Eleftherios P Diamandis and John G Marshall},
  journal={Journal of proteomics},
  year={2012},
  volume={75 4},
  pages={
          1303-17
        }
}
The proteins secreted by prostate cancer cells (PC3(AR)6) were separated by strong anion exchange chromatography, digested with trypsin and analyzed by unbiased liquid chromatography tandem mass spectrometry with an ion trap. The spectra were matched to peptides within proteins using a goodness of fit algorithm that showed a low false positive rate. The parent ions for MS/MS were randomly and independently sampled from a log-normal population and therefore could be analyzed by ANOVA. Normal… CONTINUE READING
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