Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry

  title={Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry},
  author={Hui Zhang and Xiao-Jun Li and Daniel B. Martin and Ruedi Aebersold},
  journal={Nature Biotechnology},
Quantitative proteome profiling using stable isotope protein tagging and automated tandem mass spectrometry (MS/MS) is an emerging technology with great potential for the functional analysis of biological systems and for the detection of clinical diagnostic or prognostic marker proteins. Owing to the enormous complexity of proteomes, their comprehensive analysis is an as-yet-unresolved technical challenge. However, biologically or clinically important information can be obtained if specific… 
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A critical review of analytical quantitation approaches using liquid phase separations coupled to mass spectrometry for released glycans of biopharmaceutical and biomedical significance is presented.
Solid-phase extraction of N-linked glycopeptides
A protocol for solid-phase extraction of N-linked glycopeptides and subsequent identification of N -linked glycosylation sites (N-glycosites) by tandem mass spectrometry is described.


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