Identification and purification of an adrenal medullary protein (synexin) that causes calcium-dependent aggregation of isolated chromaffin granules.


We have isolated from the adrenal medulla a protein which causes the aggregation of isolated chromaffin granules when incubated in the presence of free calcium at concentrations greater than 6 PM. The isolation procedure included precipitation in ammonium sulfate, gel filtration, and hydroxylapatite chromatography. Aggregating activity was assayed using… (More)


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