Identification and purification of actin from the subpellicular network of Toxoplasma gondii tachyzoites.

@article{AraceliPatrn2005IdentificationAP,
  title={Identification and purification of actin from the subpellicular network of Toxoplasma gondii tachyzoites.},
  author={S AraceliPatr{\'o}n and M{\'o}nica Mondrag{\'o}n and Sirenia Gonz{\'a}lez and Javier R. Ambrosio and B AlmaLGuerrero and Ricardo Mondrag{\'o}n},
  journal={International journal for parasitology},
  year={2005},
  volume={35 8},
  pages={883-94}
}
Toxoplasma gondii infects cells through dynamic events dependent on actin. Although the presence of cortical actin has been widely suggested, visualisation and localisation of actin filaments has not been reported. The subpellicular cytoskeleton network is a recently described structure possibly involved in the dynamic events. Using non-ionic detergent extractions, the cortical cytoskeleton network was enriched and used for the isolation and identification of actin. Actin was detected by… CONTINUE READING
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