Identification and purification of a protein that induces production of the Lactobacillus acidophilus bacteriocin lactacin B

@article{Barefoot1994IdentificationAP,
  title={Identification and purification of a protein that induces production of the Lactobacillus acidophilus bacteriocin lactacin B},
  author={S. Barefoot and Y. Chen and T. A. Hughes and A. Bodine and M. Shearer and M. D. Hughes},
  journal={Applied and Environmental Microbiology},
  year={1994},
  volume={60},
  pages={3522 - 3528}
}
Lactacin B is a heat-stable bacteriocin produced by Lactobacillus acidophilus N2 that is active against closely related lactobacilli, including Lactobacillus delbrueckii subsp. lactis (formerly Lactobacillus leichmannii) ATCC 4797. Pure producer cultures propagated in MRS broth (initial pH 6.5) contain no lactacin B; it is detected only in cultures maintained at pH 5.0 to 6.0 and produced optimally at pH 6.0 S. F. Barefoot and T. R. Klaenhammer, Antimicrob. Agents Chemother. 26:328-334, 1984… Expand
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References

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Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B
TLDR
Characteristics identified for lactacin B indicated that it was a peptide and confirmed its identity as a bacteriocin and its activity was bactericidal to sensitive cells and restricted to members of the family Lactobacilliaceae. Expand
Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus.
TLDR
Examination of strains of L. acidophilus within the six homology groupings of Johnson et al. demonstrated that production of the bacteriocin lactacin B could not be used in classification of neotype L. Acidophilus strains, however, the usefulness of employing sensitivity to lactac in B in classified of dairy lactobacilli is suggested. Expand
Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088
TLDR
Purification by ammonium sulfate precipitation, gel filtration, and high-performance liquid chromatography resulted in a 474-fold increase in specific activity of lactacin F. Expand
Conjugal Transfer of Plasmid-Encoded Determinants for Bacteriocin Production and Immunity in Lactobacillus acidophilus 88
TLDR
Bacteriocin production and immunity phenotypes were genetically stable in Laf Laf transconjugants not harboring pPM52 and pPM68, suggesting chromosomal integration of the transferred determinants. Expand
Plasmid-associated bacteriocin production by a Lactobacillus sake strain
Summary: A Lactobacillus sake strain L45 isolated from naturally fermented dry sausage, produced a bacteriocin designated lactocin S. The bacteriocin was moderately heat-stable and its activity wasExpand
Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481
TLDR
The data confirmed that antimicrobial activity of L. helveticus 481 was mediated by a bacteriocin, designated helveicin J, and provided evidence for a chromosomal location of helveticsin J and host immunity determinants. Expand
Characterization of a lactic acid bacterium, Carnobacterium piscicola LK5, with activity against Listeria monocytogenes at refrigeration temperatures
TLDR
Evaluation of the impact of oxygen availability, initial pH, and sodium chloride content on the effectiveness of LK5 suggested that the isolate could be used to suppress the growth of Listeria in a variety of refrigerated foods. Expand
Identification and characterization of helveticin V-1829, a bacteriocin produced by Lactobacillus helveticus 1829.
TLDR
Production of the substance was pH-dependent and maximum yields were obtained in MRS broth cultures maintained at pH 5.5, and it was bactericidal in its mode of action against indicator cells of Lact. Expand
Bacteriocin involved in premature death of Lactobacillus acidophilus NCFM during growth at pH 6.
TLDR
The results show that when L. acidophilus NCFM, a mixed strain culture, was grown at pH 6, the inhibitory strain was able to grow sufficiently to produce enough bacteriocin to destroy the predominating strain(s) in the culture. Expand
Isolation and Characterization of a Bacteriocin from a Homofermentative Lactobacillus
TLDR
Nearly 100 isolates of Lactobacillus were obtained from human and animal sources and one of these, L. helveticus strain LP27, was shown to produce a potent bacteriocin called lactocin 27, which was isolated from the culture supernatant fluid as a protein-lipopolysaccharide complex. Expand
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